Immunological responsiveness of neonatal A/J mice to isotypic determinants of syngeneic IgE.

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Immunological responsiveness of neonatal A/J mice to isotypic determinants of syngeneic IgE

We have previously shown that adult A/J mice produce high titers of anti-IgE with isotypic or idiotypic specificities in response to challenge with a conjugate of KLH with syngeneic monoclonal IgE. Thus, B cells that can synthesize anti-IgE are present in the mice. Adult mice are unresponsive to unconjugated IgE in CFA, suggesting that tolerance exists at the level of T cells. The present study...

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Induction of high titers of anti-IgE by immunization of inbred mice with syngeneic IgE.

We have generated high titers (up to 1 mg/ml) of antibodies to isotypic determinants of IgE by immunization of A/J mice with syngeneic monoclonal IgE conjugated to keyhole limpet hemocyanin. As much as 3 mg of anti-idiotypic antibodies per ml was induced at the same time. In contrast to conventional rheumatoid factors, the anti-isotypic antibodies are of moderately high affinity (10(7)-10(8) M-...

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ژورنال

عنوان ژورنال: Journal of Experimental Medicine

سال: 1988

ISSN: 0022-1007,1540-9538

DOI: 10.1084/jem.168.2.713